The goal of periodontal therapy should be either to eliminate or to reduce and maintain the number of microorganisms below the critical level. Hence the use of antibiotics and antimicrobial agents as adjuncts in the treatment of periodontal
disease
is
logical and Predictable
The purpose of the study was to evaluate the clinical and microbiological effect of delayed release pellet type doxycycline in the management of patients with adult periodontitis. Forty subjects having one site with probing pocket depths greater
than 5
mm were selected in a double-blind, randomized and placebo-controlled trial of doxycycline. Two weeks after the removal of the supragingival plaque, subjects were administrated either doxycycline at a dosage of 100 mg per 12 hours for 7 dyas, or
a
placebo. Clinical parameters of dental plaque, gingival inflammation, probing pocket depth, probing attachment level, and gingival fluid flow rate were evalutated at week 0,1,3,5 and 13. Subgingival plaque samples from each site were collected to
detect
changes tin the bacterial morphotypes under the phase contrast microscopy, and to culture aerobic and anaerobic microbes. Plaque samples were screened for the presence of Actinobacillus actinomycetemcomitans(Aa) Porphyromonas gingivalis(Pg),
Prevotella
intermedia(Pi), Fusobacterium nucleatum(Fn), Eikenella corrodens(Ec), Camphylobacter rectus(Cr) and Bacteroides forsythus(Bf) by indirect immunofluoresccnce antibody technique. the results demonstrated that doxycycline therapy showed a decrease
in
clinical parameters and proportion of spirochetes. Doxycycline therapy reduced significantly the total viable counts of anaerobic bacteria. The number of periodontopathic bacterias such as Aa, Pg, Pi, Fn, Ec, Cr, and Bf decreased significantly at
week 1
following doxycycline therapy compared to baseline data and placebo group. This trend to decrease the number of microoraganism was continued up to week. 13. These findings suggested that delayed release pellet type doxycycline may provide the
improvement of gingival health and be a effective adjunct to management of periodon topathic bacteria
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